reverse transcriptase 5 × reaction buffer (Promega)

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Promega reverse transcriptase 5 × reaction buffer
Reverse Transcriptase 5 × Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%C3%97+reaction+buffer+reverse+transcriptase/reverse+transcriptase+5+%C3%97+reaction+buffer/pmc11288740-112-39-45
Average 90 stars, based on 1 article reviews

reverse transcriptase 5 × reaction buffer - by Bioz Stars, 2026-10

90/100 stars

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Polymerase Chain Reaction:

Article Title: Empagliflozin inhibits increased Na influx in atrial cardiomyocytes of patients with HFpEF
Article Snippet: .. mRNA was extracted from right atrial biopsies using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s recommendation. cDNA was transcribed from 1 μg RNA using random primers, PCR nucleotide mix, RNasin ribonuclease inhibitor, reverse transcriptase, and reverse transcriptase 5 × reaction buffer (Promega GmbH, Walldorf, Germany) for 1 h at 37°C. mRNA Abundance of Na V 1.5 ( SCN5A ; Assay ID: Hs00165693_m1 ) and β-actin ( ACTB ; Assay ID: Hs99999903_m1) was measured using the TaqMan Gene Expression (Thermo Fisher Scientific GmbH, Dreieich, Germany) detection method. ..

Reverse Transcription:

Article Title: Non-TZF Protein AtC3H59/ZFWD3 Is Involved in Seed Germination, Seedling Development, and Seed Development, Interacting with PPPDE Family Protein Desi1 in Arabidopsis
Article Snippet: Total RNA was isolated using an RNAqueous Kit (Invitrogen, Carlsbad, CA, USA) with Plant RNA Isolation Aid (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions. .. Next, 2 μg of total RNA was reverse-transcribed in a total reaction volume of 25 μL; the reaction mixture contained 0.5 μg of oligo-dT primer, 0.5 mM dNTP, 5 μL of 5× reaction buffer, and 200 U of Moloney murine leukemia virus reverse transcriptase (Promega Corp., Madison, WI, USA). .. Quantitative RT-PCR was performed in a reaction volume of 20 μL containing 0.4 μL of cDNA, 10 μL of 2× Power SYBR Green PCR Master mix (Applied Biosystems, Foster, CA, USA), and 0.25 μM gene-specific primers.

Article Title: Evaluation of wound healing effects of ginsenoside Rg1 and red ginseng extract in STZ-induced diabetic wound model: an in vivo pilot study
Article Snippet: After precipitation with isopropanol, the RNA pellet was dissolved in distilled water treated with diethyl pyrocarbonate (DEPC; WR2004; Biosesang, Sungnam, Korea). .. Total RNA was quantified to 3 μg to react 1 μg/μL random hexamer (C1181; Promega, Madison, WI, USA) at 70°C for 5 min, then incubated at 4°C for 5 min. Each reaction contained 1 μL of 10 mM dNTPs, 1 μmol/L MgCl 2 , 1 μg/μL RNaseOUT, 1 μg/μL 5×reaction buffer, 1 μL GoScript reverse transcriptase (A5003; Promega), and 7 μL RNase-free double-distilled H2O (N2511; Promega) and was incubated at 25°C for 5 min, followed by incubation at 42°C for 1 h and 70°C for 15 min. ..

Article Title: Pinealectomy increases thermogenesis and decreases lipogenesis
Article Snippet: Total RNA was extracted with Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. For RT-PCR, 5 μg of total RNA were reverse transcribed for 30 min at 37°C in a reaction mixture containing RNA, 40 U RNase inhibitor (Promega, Madison, WI, USA), 0.5 mM deoxynucleotide triphosphate (Promega, Madison, WI, USA),2 μM random hexamer primers, 5 x AMV reverse transcriptase reaction buffer and 30 U AMV reverse transcriptase (Promega, Madison, WI, USA). .. Real-time RT-PCR analysis was performed using the SYBR Green PCR Master Mix (TOYOBO, Osaka, Japan) and real-time RT-PCR parameters were used as follows: initial denaturation 95°C for 30 s, amplification 45 cycles were performed at 95°C for 5 s, 60°C for 10 s, and 72°C for 15 s, melting curve 95°C for 10 s, 65°C for 1 min, cooling 37°C for 10 min with gene-specific primers.

Article Title: Non-cytotoxic doses of shikonin inhibit lipopolysaccharide-induced TNF-α expression via activation of the AMP-activated protein kinase signaling pathway
Article Snippet: Briefly, total RNA was extracted from the cells using TRIzol ® reagent (cat. no. 15596018; Invitrogen; Thermo Fisher Scientific, Inc.). .. Then, the extracted RNA (2 μg; 10 μl) was reverse transcribed using AMV reverse transcriptase (cat. no. M5101; Promega Corporation) in a 25 μl final reaction volume containing AMV Reverse Transcriptase (3 μl), AMV Reverse Transcriptase 5X Reaction Buffer (5 μl; cat. no. M5101; Promega Corporation), 10 mM dNTP (2.5 μl; cat. no. U1330; Promega Corporation), RNasin ® Ribonuclease Inhibitor (1 μl; cat. no. N2511; Promega Corporation), 500 μg/ml oligo(dT) 15 primer (2 μl; cat. no. C1101; Promega Corporation) and nuclease-free water (1.5 μl; cat. no. P1193; Promega Corporation), which were incubated at 42 ̊C for 60 min. qPCR reactions were carried out on an ABI Prism 7500 RT PCR instrument (Applied Biosystems; Thermo Fisher Scientific, Inc.) in triplicate using SYBR Premix Ex Taq (cat. no. RR420A; Takara Bio Inc.). ..

Article Title: Comparative Transcriptomic and Molecular Pathway Analyses of HL-CZ Human Pro-Monocytic Cells Expressing SARS-CoV-2 Spike S1, S2, NP, NSP15 and NSP16 Genes
Article Snippet: Extracted RNA was subjected to conventional reverse transcription (RT) for first-strand cDNA synthesis by mixing 0.5 μL of 500 ng/μL random hexamers (Promega, Madison, WI, USA), 1 μL of RNA (500 ng), and 2.25 μL of nuclease-free water to achieve a total volume of 3.75 μL. .. The mixture was then heated to 70 °C for 5 min before incubating on ice for 1 min. A total of 1.25 μL of MMLV reverse transcriptase 5× reaction buffer (Promega), 1.25 μL of dNTPs (10 μM), 0.16 μL of recombinant RNasin ribonuclease inhibitor (Promega), 0.25 μL of MMLV reverse transcriptase (Promega), and nuclease-free water were added to the mixture to give a final total reaction volume of 10 μL, and incubated at 37 °C for 1 h. Following first-strand synthesis, the cDNAs were diluted five times with nuclease-free water. .. Real-time PCR was then carried out for each sample using 5 μL of FastStart Essential DNA Green Master (Roche, Basel, Switzerland), 3 μL of nuclease-free water, 0.5 μL of target gene forward primer (10 μM), 0.5 μL of target gene reverse primer (10 μM) , and 1 μL of diluted cDNA.

Gene Expression:

Virus:

Random Hexamer:

Incubation:

Real-time Polymerase Chain Reaction:

Reverse Transcription Polymerase Chain Reaction:

other:

Article Title: MUC21 induces the viability and migration of glioblastoma via the STAT3/AKT pathway
Article Snippet: Total RNA was reverse transcribed into cDNA at 42 ̊C for 1 h using M-MLV reverse transcriptase (cat. no. M1701; Promega Corporation, includes M-MLV 5X Reaction Buffer 5 μl, dNTP, 10 mM 1.25 μl, Recombinant RNasin ® Ribonuclease Inhibitor 25 units, M-MLV RT 200 units and Nuclease-Free Water to final volume of 25 μl). qPCR was then performed using the SYBR Ex Taq kit (cat. no. 638319; Takara Bio, Inc.) and used according to the manufacturer's protocol.

Article Title: Rapid display method in translational synthesis of peptide
Article Snippet: Then, the washed magnetic beads were suspended in 10 μL of a reverse transcription reaction solution (5 units/μL M-MLV Reverse Transcriptase (Promega), 2 μM CGS3an21.R44, 0.5 mM dNTP, 10 mM Tris-HCl (pH 8.3), 15 mM potassium chloride, 0.6° C. mM magnesium chloride, 2 mM dithiothreitol), and the suspension was warmed at 42° C. for 1 hr to perform reverse transcription reaction.

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